Multiple airy beams light-sheet fluorescence microscopy

Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field view benefiting from its non-diffracting nature, but cost out-of-focus background and low contrast caused by side lobes illumination. Here, we propose method to generate sort multiple beams (MAB), which are linearly superimposed with different scale factors. Compared SAB light-sheet, energy more concentrated on focal plane detection objective, improve decrease photodamage effect. Furthermore, combined complementary subtraction (CBS) strategy increase axial resolution, termed as beams-complementary method, enables resolution 1.2 μm while keeping 450 × μm. The effectiveness demonstrated fluorescent beads aspergillus conidiophores.